Ficoll Flotation for the Separation of Blood Leukocyte Types
نویسندگان
چکیده
منابع مشابه
Ficoll flotation for the separation of blood leukocyte types.
T SHOULD BE POSSIBLE to separate cells of different types by utilizing differences in their specific gravities. Centrifugation of whole blood against high density fluids has been successful, at least in part, in separating leucocytes from peripheral blood.15 Gum acacia3 and bovine albumin1’2’5 have been used in this manner, but the method has been criticized because of the cost and labor involv...
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Separation of avian heterophils from blood using Ficoll-Hypaque discontinuous gradients.
Rapid separation of avian heterophils from anticoagulated whole blood was achieved using Ficoll-Hypaque discontinuous gradients. An average of 14.4% of blood heterophils was harvested with a mean purity exceeding 99%. Heterophil viability, as determined by trypan blue dye exclusion, averaged 99.8%. The integrity of isolated heterophils was evaluated by cytochemical staining and ultrastructural ...
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ژورنال
عنوان ژورنال: Blood
سال: 1968
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v31.1.66.66